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KMID : 0620920110430040205
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Experimental & Molecular Medicine
2011 Volume.43 No. 4 p.205 ~ p.215
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Anti-obesity effects of Lysimachia foenum-graecum characterized by decreased adipogenesis and regulated lipid metabolism
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Seo Jong-Bae
Choe Sung-Sik
Jeong Hyun-Woo
Park Sang-Wook
Shin Hyun-Jung
Choi Sun-Mi
Park Jae-Young
Choi Eun-Wook
Kim Jae-Bum
Seen Dong-Seung
Jeong Jae-Yeon
Lee Tae-Gyu
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Abstract
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Lysimachia foenum-graecum has been used as an oriental medicine with anti-inflammatory effect. The anti- obesity effect of L. foenum-graecum extract (LFE) was first discovered in our screening of natural product extract library against adipogenesis. To characterize its anti-obesity effects and to evaluate its potential as an anti-obesity drug, we performed various obesity- related experiments in vitro and in vivo. In adipogenesis assay, LFE blocked the differentiation of 3T3-L1 preadipocyte in a dose-dependent manner with an IC50 of 2.5 μg/ml. In addition, LFE suppressed the expression of lipogenic genes, while increasing the expression of lipolytic genes in vitro at 10 μg/ml and in vivo at 100 mg/kg/day. The anti-adipogenic and anti- lipogenic effect of LFE seems to be mediated by the inhibition of PPARγ and C/EBPα expression as shown in in vitro and in vivo, and the suppression of PPARγ activity in vitro. Moreover, LFE stimulated fatty acid oxidation in an AMPK-dependent manner. In high-fat diet (HFD)-induced obese mice (n = 8/group), oral administration of LFE at 30, 100, and 300 mg/kg/day decreased total body weight gain significantly in all doses tested. No difference in food intake was observed between vehicle- and LFE-treated HFD mice. The weight of white adipose tissues including abdominal subcutaneous, epididymal, and perirenal adipose tissue was reduced markedly in LFE-treated HFD mice in a dose-dependent manner. Treatment of LFE also greatly improved serum levels of obesity-related biomarkers such as glucose, triglycerides, and adipocytokines leptin, adiponectin, and resistin. All together, these results showed anti-obesity effects of LFE on adipogenesis and lipid metabolism in vitro and in vivo and raised a possibility of developing LFE as anti-obesity therapeutics.
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KEYWORD
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adipocyte differentiation, fatty acid oxidation, fatty acid synthesis, lipid metabolism, Lysimachia foenum-graecum, obesity
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